Thymosin alpha-1 (Tα1) peptide: sequence, how it is supplied, COA, sizes

Thymosin alpha-1 (Tα1) is a peptide of 28 amino acids first isolated in 1977 from calf thymus by Allan Goldstein's group, who had been fractionating thymic extracts for a decade.

It later turned out to be the N-terminal fragment of a larger protein, prothymosin alpha, and to carry an acetyl group on its N-terminus, a feature that matters on a certificate.

The same sequence has been produced synthetically since the 1980s and is licensed in several countries under the trade name Zadaxin.

What is sold as thymosin alpha-1 for research is the identical 28-residue acetylated peptide, made by solid-phase synthesis, purified by preparative HPLC and freeze-dried. Research use only. Not for human or veterinary use.

Three catalog entries carry the word "thymosin" or "thym-", and buyers regularly ask for one while meaning another. They are three different objects.

The alpha and beta thymosins are unrelated sequences that happen to have been isolated from the same preparation of the same tissue (thymosin fraction 5, a calf-thymus extract first prepared in the 1960s), which is the only reason they share a name.

Thymalin is the crude fraction they were isolated from.

A listing that describes thymosin alpha-1 with the properties of TB-500, or that gives thymalin a single molecular mass, has confused the three. The seven-residue peptide has its own guide: TB-500 peptide: sequence, COA, sizes.

Further reading: epithalon, another short synthetic peptide and TB-500 buying guide.

The N-terminal serine of thymosin alpha-1 carries an acetyl group (CH₃CO-), added during synthesis as the last step on the resin. It changes three things a certificate reports.

• Mass. The acetyl adds 42.0 daltons. A "thymosin alpha-1" whose mass reconstructs to 3,066.3 instead of 3,108.3 was never acetylated and is the des-acetyl peptide, a different molecule.
• HPLC. The acetylated peptide has no free N-terminal amine, so it elutes slightly later than the des-acetyl form on reverse phase. An incomplete acetylation shows as a small peak just before the main one; a well-made lot shows it below 0.5 %.
• Stability. Without a free amine at the N-terminus, the peptide is not a substrate for aminopeptidases and does not undergo the N-terminal side reactions (diketopiperazine formation) that shorten the shelf life of some sequences. On the shelf it is one of the better-behaved peptides of its size.

The sequence contains no methionine, cysteine or tryptophan, so oxidation is not the concern it is for sermorelin.

Its nine acidic residues do make it strongly hygroscopic as an acetate salt, which is why a sealed vial matters more for this peptide than for most.

HPLC purity: one main peak

On reverse-phase HPLC the peptide elutes as a single main peak; the purity figure is the area of that peak against all peaks.

The impurities to look for are the des-acetyl peptide (eluting just before), deletion sequences from synthesis (a chain missing one residue) and, for a peptide with so many glutamate and aspartate residues, aspartimide-related by-products that elute close to the main peak.

Mass spectrometry: 3,108.3

A peptide of this size and charge is seen by electrospray ionization as multiply charged ions, in negative mode as readily as in positive.

Some laboratories include the observed ions and the deconvoluted mass on the report.

For thymosin alpha-1 the expected values in positive mode are roughly m/z 1,037.1 for [M+3H]³⁺ and 778.1 for [M+4H]⁴⁺, both reconstructing to 3,108.3. A deconvoluted 3,066 is the des-acetyl form.

Net peptide content, water, endotoxin

Net content (by amino-acid analysis or UV) tells how many of the labeled milligrams are peptide; with only four basic residues to carry acetate counter-ions, 85 to 90 % is typical for this sequence, higher than for a basic peptide.

Water (Karl Fischer) is typically 3 to 8 % in a lyophilized cake and rises fast in an opened vial of this hygroscopic salt. Endotoxin (LAL, in EU/mg) says whether the water and glassware of the fill were clean.

What else must be on the page

• The laboratory's name and a verifiable test number (Janoshik Analytical is the reference in this market).
• The lot number, with the task number and verification key the laboratory confirms.
• The test date and a photograph of the tested vial wearing the supplier's label. A certificate for a bare manufacturer's vial says nothing about the finished product.

The OXpeptides Janoshik reports (Retatrutide and GHK-Cu) are in the COA library; the thymosin alpha-1 product page sets out the sequence, the vial size and the live price. The field-by-field method is in how to read a peptide COA.

Further reading: ARA-290 data sheet, BPC-157 vs TB-500 compared and Wolverine peptide.

Thymosin alpha-1 is stocked in one size, 10 mg: 10 mg of a 3.1 kDa peptide is 3.2 micromoles. The catalog card at the top of this page reads the live price. Lot prices apply automatically from 3 and from 5 vials of the same size.

What the price includes at OXpeptides: a sealed, labeled vial and the lyophilized compound inside, named with its net quantity in mg on the label. Ships from the EU · tracked: €9.90, free from €120.

See thymosin alpha-1 in the catalog →

• Temperature: −20 °C for long-term storage, in the sealed vial, away from light and moisture.
• Moisture: the point to watch for this peptide. Nine acidic residues make the acetate salt strongly hygroscopic; keep the vial sealed and in its box, and let a cold vial reach room temperature before it leaves its bag so no water condenses on the glass.
• Light and oxygen: no oxidizable residue in the sequence; the usual closed box is enough.
• Shelf life: 24 months for a sealed lyophilized vial stored cold; the date on the label governs.

A few days of ambient shipping do not degrade the sealed powder. The rules compound by compound are in lyophilized peptides: storage and shelf life.

"Thymosin alpha 1" returns encyclopedic pages, journal articles and clinics before it returns a research supplier. For a laboratory that needs the reagent, the listing that matters answers five questions on the page itself.

1. Is it the right molecule? 28 residues, acetylated, 3,108.3 g/mol. Not TB-500 (889), not thymalin (a mixture).
2. Is there a batch COA from a named independent laboratory, verifiable on the lab's site, with HPLC purity, the mass at 3,108.3 and net peptide content?
3. Does the COA show the supplier's own labeled vial, and does it carry a task number and verification key the laboratory confirms?
4. Does the vial ship from domestic stock, tracked, in a few days? The catalog ships from our EU stock, tracked, to the European Union.
5. Does the page describe the reagent only (sequence, purity documentation, storage), with "research use only" stated plainly? A supplier that publishes usage content is a supplier that is easy to shut down, and that risk lands on the buyer whose parcel is in transit.

The seven-point method is in how to check a peptide supplier; the Europe-wide checklist in where to buy research peptides in Europe.

Further reading: BPC-157 buying guide.