CJC-1295 DAC, Mod GRF 1-29 and the CJC-1295 / Ipamorelin blend: what is in each vial

The name "CJC-1295" covers two different peptides, and "CJC-1295 / Ipamorelin" adds a third. The certificate of analysis tells them apart by mass; the label alone does not.

The unmodified parent sequence, GHRH 1-29 itself, is sold as sermorelin and has its own guide: sermorelin peptide: sequence, COA, sizes.

All three are made by solid-phase synthesis, purified by preparative HPLC and supplied as lyophilized acetate salts. Research use only. Not for human or veterinary use.

The DAC is a small chemical tag, a maleimidopropionic acid attached to a lysine added at position 30.

It adds 279.4 daltons to the 29-residue analogue, which is the whole difference between 3,367.9 and 3,647.3 g/mol.

On a mass spectrum the two are impossible to confuse: 3,647 is CJC-1295 DAC, 3,368 is Mod GRF 1-29, whatever the label says.

Two further details identify the DAC version on a report. First, its HPLC retention time is longer, because the linker is hydrophobic.

Second, the maleimide group is reactive toward thiols; a laboratory that stores the reference standard in a thiol-containing buffer will see an adduct peak.

Neither is a defect of the reagent, but both are reasons the DAC and no-DAC versions cannot be certified against the same standard.

The catalog keeps the two apart on purpose: CJC-1295 DAC is sold alone in a 5 mg vial, and the no-DAC sequence is sold only inside the blend, where the certificate reports it under its own name, Mod GRF 1-29.

The blend is one lyophilized vial holding both peptides in equal mass, weighed separately and freeze-dried together.

Equal mass does not mean equal molar amount.

Mod GRF 1-29 is 3,368 g/mol and Ipamorelin 712 g/mol, so 5 mg of each is 1.5 micromoles of the GHRH analogue against 7.0 micromoles of the pentapeptide, a molar ratio of about 1 : 4.7.

A certificate reports the two masses, and a buyer who needs the molar ratio derives it from them.

Both components are white powders, so the cake is white; there is no color test as there is for a copper-peptide blend. The label states both names, the total quantity and "research use only".

The blend uses the no-DAC analogue because a linker designed to hold a peptide in circulation for days makes no sense next to a pentapeptide that is cleared in minutes; a "CJC-1295 DAC / Ipamorelin" blend is a listing to question.

Further reading: AOD-9604 data sheet, the Wolverine BPC-157 / TB-500 blend and TB-500 data sheet.

HPLC: two main peaks, two purity figures

On reverse-phase HPLC the pentapeptide elutes early and the 29-residue analogue late; the chromatogram shows two well-separated main peaks.

The laboratory identifies each against its reference standard and reports a purity figure per component, from the area of that component's peak against its own impurities.

A certificate that prints one "99 %" for the vial has averaged two numbers into one and hides which component is under specification.

Mass spectrometry: two expected masses

Ipamorelin is seen as the singly charged ion at m/z 712.4 [M+H]⁺ (monoisotopic), and Mod GRF 1-29 as multiply charged ions (about 1,123.6 for [M+3H]³⁺, 843.0 for [M+4H]⁴⁺) reconstructing to 3,367.9.

Some laboratories offer this spectrum as an option; when it is run, both masses must be present, and 3,647 in a "no DAC" blend means the DAC version was used.

What else must be on the page

• The laboratory's name and a verifiable test number (Janoshik Analytical is the reference in this market).
• The lot number, with the task number and verification key the laboratory confirms.
• The test date and a photograph of the tested vial wearing the supplier's label.

At OXpeptides, the Janoshik reports for Retatrutide and GHK-Cu are published in the COA library; the blend product page sets out the sequence, the vial size and the live price.

The full reading method is in how to read a peptide COA; a four-peptide example is in the KLOW guide.

Three listings cover the three molecules:

• CJC-1295 / Ipamorelin blend: 10 mg (5 + 5). One laboratory test covers both components.
• CJC-1295 DAC: 5 mg, alone.
• Ipamorelin: 5 mg, alone, for a laboratory that prefers to hold each compound in its own lot.

The catalog card at the top of this page reads the live prices.

Bought as two separate vials, 5 mg of each peptide costs more than the 10 mg blend, because two vials mean two fills and two labels.

Volume tiers apply from two vials of the same size; the subscription price applies to every delivery.

What the price includes at OXpeptides: a sealed, labeled vial and the lyophilized compound inside, named with its net quantity in mg on the label. Ships from the EU · tracked: €9.90, free from €120.

See the CJC-1295 / Ipamorelin blend in the catalog →

• Temperature: −20 °C for long-term storage, in the sealed vial, away from light and moisture.
• Light, moisture, oxygen: keep the vial sealed and in its box; let a cold vial reach room temperature before it leaves its bag.
• The DAC linker: the maleimide group is the one part of these three molecules that reacts with something other than water. Keep the CJC-1295 DAC vial sealed and away from any thiol-containing reagent until it is used.
• Shelf life: 24 months for a sealed lyophilized vial stored cold; the date on the label governs.

A few days of ambient shipping do not degrade the sealed powder. The rules compound by compound are in lyophilized peptides: storage and shelf life.

1. Which CJC-1295? The page must say DAC or Mod GRF 1-29, and the certificate must show 3,647 or 3,368.
2. For a blend, are both masses stated (5 + 5) and does the COA carry two peaks and two masses?
3. Is the COA from a named independent laboratory, verifiable on the lab's site, showing the supplier's labeled vial, with a task number and verification key the laboratory confirms?
4. Does the vial ship from domestic stock, tracked, in a few days? The catalog ships from our EU stock, tracked, to the European Union.
5. Does the page describe the reagent only, with "research use only" stated plainly?

The seven-point method is in how to check a peptide supplier; the Europe-wide checklist in where to buy research peptides in Europe.

Further reading: Selank buying guide, MOTS-c buying guide and Semax buying guide.

Sermorelin is the first 29 residues of growth-hormone-releasing hormone (GHRH 1-29), 3,357.9 g/mol. Mod GRF 1-29, sold as "CJC-1295 without DAC", is the same chain with four amino acids replaced. Each change has a chemical reason:

Mod GRF 1-29 weighs 3,367.9 g/mol, 10 Da more than sermorelin.

That difference is small, but a mass spectrum resolves it, and it is the only way to tell from a certificate whether a vial holds sermorelin or Mod GRF 1-29. The parent peptide has its own data sheet.

CJC-1295 with DAC is Mod GRF 1-29 with one more residue at the end: a lysine carrying a maleimidopropionyl group, the DAC (an affinity complex).

A maleimide reacts with free thiol groups, and the most accessible one in blood plasma is cysteine 34 of serum albumin. The design goal of the DAC is to attach the peptide covalently to albumin.

For a laboratory, what matters is that DAC and no-DAC are two different molecules:

• Mass: 3,647.3 g/mol with DAC against 3,367.9 without, about 280 Da apart.
• Length: 30 residues against 29.
• Reactivity: the maleimide is reactive by design, so the DAC version is more sensitive to moisture and to thiol-containing buffers.

Listings that say "CJC-1295" without qualification are ambiguous. The mass on the certificate removes the ambiguity in one line.

Ipamorelin is a pentapeptide: Aib-His-D-2-Nal-D-Phe-Lys-NH₂, formula C₃₈H₄₉N₉O₅, 711.9 g/mol. Three of its residues are not among the twenty standard amino acids or not in their usual form:

• Aib (α-aminoisobutyric acid) at position 1, a residue with two methyl groups on the same carbon;
• D-2-Nal (D-2-naphthylalanine) at position 3, a large aromatic side chain in the D form;
• D-Phe at position 4, phenylalanine in its mirror-image form.

The C-terminus is an amide.

These features make ipamorelin a small, compact molecule with strong UV absorbance from its two aromatic rings, which helps the HPLC reading.

It is about a fifth of the mass of Mod GRF 1-29, which matters as soon as the two share a vial.

The blend vial holds 5 mg of Mod GRF 1-29 and 5 mg of ipamorelin. Equal mass does not mean equal numbers of molecules:

There are almost five ipamorelin molecules for every Mod GRF 1-29 molecule.

Laboratories that work in molar terms need this figure, and it is one more reason to keep each component's certificate line: the quantity of each peptide, not of the blend, is what counts.

On the mass spectrum, the two give very different signals. Ipamorelin shows a singly charged ion near 712.9.

Mod GRF 1-29, a 29-residue chain with several basic residues, appears mostly as multiply charged ions, around 1,123.6 for [M+3H]³⁺ and 843.0 for [M+4H]⁴⁺.

A certificate that identifies both, each with its own purity, is the minimum for a two-peptide vial.

The names in this family are used loosely, and the mismatch usually shows on the label before it shows on the certificate:

• "CJC-1295" alone: could be the DAC version or Mod GRF 1-29. Ask which, then check the mass.
• "CJC-1295 no DAC" and "Mod GRF 1-29": the same molecule under two names, 3,367.9 g/mol.
• "GHRH" or "GRF" without a modification: usually sermorelin, 3,357.9 g/mol.
• "CJC/Ipa 10 mg" without a split: the label should state how the 10 mg divide between the two peptides.

The rule is the same for every listing: the compound named precisely, the quantity per component, a certificate for that lot that prints the observed masses. Research use only. Not for human or veterinary use.

A certificate for the 10 mg blend vial has to answer three questions, one line each:

1. Which two molecules are in the vial? Each peak of the HPLC trace is identified by mass spectrometry: Mod GRF 1-29 near 3,367.9 g/mol, ipamorelin near 711.9 g/mol. A certificate that names "CJC-1295" without a mass leaves the DAC question open.
2. How pure is each one? The purity is the area of each main peak against the impurities that belong to it, not one figure for the whole trace. Two short-chain impurities under the ipamorelin peak and one oxidised form under the Mod GRF 1-29 peak are three different things.
3. How much of each is there? A quantity per component, close to 5 mg each, is what turns "10 mg blend" into a measured figure.

The two peaks are far apart on most reversed-phase methods: ipamorelin, small and aromatic, does not behave like a 29-residue chain.

That makes the blend easier to read than a mixture of two similar peptides, provided the laboratory reports both.

Four molecules, five product names. The only reliable key between them is the mass printed on the certificate of the lot.

Freeze-drying two peptides together fixes their ratio for the life of the vial. A laboratory that needs another ratio, or one compound without the other, cannot separate them again.

Two separate vials keep that freedom and give two independent certificates, at the cost of a second vial in the order. The catalog lists CJC-1295 and ipamorelin on their own as well, next to the blend.

The same logic applies to every blend on the market, from the BPC-157 / TB-500 wolverine blend to KLOW: the blend saves a step, the separate vials keep control. Research use only. Not for human or veterinary use.