Bacteriostatic water: what it is, sizes, where to buy it for a laboratory

Bacteriostatic water is water that has been sterilized and to which 0.9 % benzyl alcohol (9 mg per mL) has been added as a preservative.

"Bacteriostatic" describes what the benzyl alcohol does: it stops bacteria from multiplying inside the vial.

It does not sterilize anything on contact — the water is sterile because it was filled that way, and the preservative keeps it that way in an opened vial.

The United States Pharmacopeia (USP) describes this category of water; a vial that says "USP" on its label is stating that it was prepared to that monograph.

Nothing else is in the vial: no salts, no buffer, no dextrose. The pH is typically between 4.5 and 7.0.

In a laboratory it is the standard solvent for lyophilized compounds — the powder in the peptide vials of this catalog. It is sold and described here as a laboratory supply only. Research use only. Not for human or veterinary use.

The two are often confused because both are clear, sterile and sold in similar vials. The difference is the preservative.

"Bacteriostatic water for peptides" and "bac water" are the names laboratories give the same product when they order it alongside lyophilized peptides.

Nothing changes in the vial: it is the sterile, preserved water described above.

What changes is the lot discipline around it — a research supplier lists it with the same label requirements as its reagents (concentration, volume) and ships it in the same parcel, so the solvent and the powders it accompanies arrive together, from the same source.

The catalog lists it next to every lyophilized compound for that reason. See bacteriostatic water in the catalog.

Three sizes cover the market. The right one is a question of how many vials of powder a laboratory works through, not of price per milliliter.

• 3 mL — the size sold in this catalog. One small vial, one lot. See bacteriostatic water in the catalog.
• 10 mL — the traditional laboratory size, a glass vial with a rubber stopper and crimp cap.
• 30 mL — the size behind the "bacteriostatic water 30ml" search. Made by a small number of manufacturers and periodically out of stock across the whole market, which is why the search volume for it is high.

Whatever the size, the label must carry the concentration of benzyl alcohol and the volume, and the lot must come with its certificate. A bottle with no lot number is not a laboratory supply.

Bacteriostatic water is inexpensive, and that is exactly why the market is full of unlabeled or re-bottled vials. Five checks:

1. The label says "0.9 % benzyl alcohol" and "USP". If the concentration is missing, so is the guarantee.
2. Sealed crimp cap, intact septum. A vial that arrives with the aluminum seal removed or a punctured septum is not sterile.
3. A printed label, not hand-written, and a certificate for the lot.
4. Clear, particle-free liquid. Cloudiness, floating particles or a yellow tint mean the vial goes in the bin.
5. A supplier that sells it as a laboratory supply, with the same lot discipline as its reagents — not a marketplace seller with a stock photo.

The same rules apply to every product in the catalog; they are laid out in where to buy research peptides in Europe.

The USP monograph, the certificate of a water lot and the makers of each size are covered in bacteriostatic water for research: the laboratory guide.

Local retail rarely stocks bacteriostatic water for walk-in customers, and when it does it is the 30 mL size at a hospital-supply price.

Online laboratory suppliers are where the 3 and 10 mL vials are, shipped in a few days with the lot number visible on the listing.

Two things a local purchase does not give you and a research supplier does: the vial ships with the peptides it is meant to accompany, in the same parcel and under the same lot discipline, and the listing tells you the manufacturer and the concentration before you pay.

Bacteriostatic water is one of the few items in a research order that does not go in the freezer.

• Temperature: 15–25 °C (room temperature). Do not freeze; ice can crack the glass and freezing alters the preservative's distribution.
• Light: keep the vial in its box. Benzyl alcohol is light-sensitive over long periods.
• Shelf life: 24 months for a sealed vial kept under these conditions.

The storage rules for the powders it accompanies are different — cold, dark, dry — and are described in lyophilized peptides: storage and shelf life.

Bacteriostatic water is a low-cost item; the price on the listing matters less than the label on the vial.

The catalog sells a 3 mL sealed vial, 0.9 % benzyl alcohol, labeled with the concentration and the volume. The live table at the top of this page shows the current price.

See bacteriostatic water in the catalog →