Janoshik test report: how to read one, and how to verify it

Janoshik Analytical is an independent analytical laboratory in the Czech Republic that accepts samples from anyone and returns a one-page report. It has no stake in any catalogue.

Over the last few years it became the address peptide suppliers send their lots to, for two plain reasons: it accepts small submissions, and it publishes each report at a stable address, so a buyer can check the document without going through the supplier.

That second point is the whole argument.

A certificate that exists only as a JPEG on a shop page proves nothing, because a JPEG can be edited in a minute.

A report that carries a number the laboratory itself will confirm is a different object: to forge it you would have to forge the laboratory.

Janoshik is not the only laboratory doing this work.

Freedom Diagnostics, Colmaric Analyticals and ILS run comparable programmes, and a large manufacturer will often hold an in-house certificate as well.

The reading method below applies to all of them; the field names move, the questions do not.

A report is short on purpose. Every line is a fact that can be checked, and a line that is missing is a fact nobody wanted to state.

The field-by-field reading of a full certificate, including the fields Janoshik does not carry, is in how to read a peptide COA.

Reverse-phase HPLC: how clean

The sample is dissolved, pushed through a column and detected by UV at 214 nm, the wavelength of the peptide bond.

Each compound leaves the column at its own time and draws a peak. Purity is the area of the main peak divided by the area of everything that appeared, expressed as a percentage.

What this settles: the sample is one compound rather than a mixture. What it does not settle: which compound.

A 99.4 % figure on a vial of the wrong peptide is a very clean vial of the wrong peptide. The arithmetic behind the percentage, and why 99 % is a weaker claim than it sounds, is in what 99 % purity actually means.

Mass spectrometry: which molecule

The dissolved sample is ionized and its mass measured. That measured mass is compared with the mass computed from the declared sequence.

A GHK‑Cu sample must answer near 340.4 for the free tripeptide; a TB‑500 sample near 889.0; a 15-residue BPC‑157 sample near 1,419.5.

A difference of one residue shows up immediately, and so does a sequence built in the wrong order only if the laboratory also runs a sequencing step, which a standard report does not.

What this settles: identity, to the nearest mass. What it does not settle: stereochemistry, or two sequences that happen to share a mass.

Net peptide content: how much you actually bought

A lyophilized peptide is an acetate salt and carries residual water. A vial filled with 10 mg of powder typically holds 8 to 9 mg of peptide.

Net content, measured by amino-acid analysis or by nitrogen determination, states that figure.

A laboratory computing a concentration divides by it rather than by the label, which is why the bacteriostatic water calculator ends with a caveat about the label mass.

A report that gives purity and mass but not net content is still a real report. It simply leaves the most expensive question open.

Four steps, and none of them require trusting the shop page the document was found on.

1. Read the test number at the top of the page, and type it into the laboratory own lookup rather than clicking a link on the shop page. A link can point anywhere; an address typed by hand cannot.
2. Compare what comes back with the page you were shown: the same compound, the same date, the same purity figure. A number that opens a different document, or nothing at all, ends the conversation.
3. Match the lot on the report against the lot printed on the vial in hand. This is the step almost nobody does, and it is the one a re-used certificate cannot survive.
4. Look at the photograph. If the vial in the picture wears the supplier label and the same lot, the document describes the finished product. A bare vial, or a label from another brand, describes something else.

Where a supplier publishes the report matters as much as whether it has one.

A certificate on the product page, dated, with a lot number, beats a promise to send one on request every time. OXpeptides publishes them per lot in the COA library and on each product page the day they come back.

Outright forgery is rare, because it is easy to catch. What circulates instead is real documents used dishonestly.

1. The manufacturer certificate. A genuine report on a bare vial, submitted by the factory, shown as though it described the reseller finished product. Recognised by the photograph: no label, or a label in another alphabet.
2. The frozen certificate. One report from 2023 still on the page in 2026, under four different lots. Recognised by the date, and by a lot number that never changes.
3. The cropped page. The purity figure kept, the test number and the sample name cropped away. Recognised by what is missing.
4. The good-lot submission. A supplier submits the cleanest vial of a run and sells the rest. Nothing on the page reveals this, which is why a certificate per lot, not per product, is the thing to ask for.
5. The average on a blend. A four-peptide vial reported as a single purity figure. A blend must carry one figure per component, one peak per component. The case of the KLOW blend shows what a correct blend report looks like.

A report is a statement about one sample on one day. Reading it as a statement about a company is where buyers get hurt.

• It says nothing about the next lot. Purity is a property of a production run, not of a brand. A supplier who published a fine report in March owes you a fresh one for the vial shipped in September.
• It says nothing about storage or shipping. A sealed lyophilized vial is stable, and a report issued before a summer in a warehouse describes the powder as it was, not as it is. The storage rules are in lyophilized peptides: storage and shelf life.
• It says nothing about sterility or endotoxin. Those are separate analyses, ordered separately, and a purity report does not include them.
• It says nothing about anything living. A purity figure is chemistry. Research use only. Not for human or veterinary use.

Every lot goes to an independent third-party laboratory.

The certificate is published in the COA library and on the product page the day it comes back, with the lot number, the purity figure and the date.

Until a lot has been analysed, the entry reads analysis pending, never an estimate and never a figure borrowed from a previous run.

The catalogue does not name the laboratory on a page before a signed report exists to name it on, which is the same rule applied to purity: the product pages carry the specification (>99 % by HPLC) and the measured figure lives on the certificate.

The copper tripeptide sold in 100, 200 and 300 mg sizes is on the GHK‑Cu page; the seven-point method for judging any supplier, certificate included, is in how to check a peptide supplier.